https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41140 3O₄ nanoparticles (NP) received great attention due to their effective peroxidase like activity. The pH dependent peroxidase activity of Fe₃O₄ NP results in generation of radical ^·OH radical via the unique Fenton chemistry of iron. However, their pH dependent activity is restricted to acidic environment and dramatic loss in antibacterial activity is observed at near neutral pH. Here we describe a novel strategy to overcome the pH lacunae of citrate coated Fe₃O₄ NP by utilizing adenosine triphosphate disodium salt (ATP) as a synergistic agent to accelerate the radical ^·OH radical production and restore its antibacterial activity over a wide range of pH. This synergistic combination (30 µg/mL Fe₃O₄ NP and 2.5 mM ATP) shows a high bactericidal activity against both gram positive (B. subtilis) and gram negative (E. coli) bacterial strains, in presence of H₂O₂, at neutral pH. The synergistic effect (Fe₃O₄ NP + ATP) is determined from the viability assessment and membrane damage studies and is further confirmed by comparing the concentration of generated radical ^·OH radicals. Over all, this study illustrates ATP assisted and radical dotOH-mediated bactericidal activity of Fe₃O₄ nanozyme at near neutral pH.]]> Wed 27 Jul 2022 14:33:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31345 Escherichia coli to produce [ᴅ-Asp³]microcystin-LR and microcystin-LR. We assembled a 55 kb hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster from Microcystis aeruginosa PCC 7806 using Red/ET recombineering and replaced the native promoters with an inducible Ptet_O promoter to yield microcystin titers superior to M. aeruginosa. The expression platform described herein can be tailored to heterologously produce a wide variety of microcystin variants, and potentially other cyanobacterial natural products of commercial relevance.]]> Wed 24 Nov 2021 15:51:49 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26208 Wed 11 Apr 2018 15:30:25 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34252 mcr-1 gene on incompatibility group IncI2 plasmids in Escherichia coli isolated from patients in New South Wales, Australia, in 2011 and 2013. mcr-1 is present in a small minority of colistin-resistant Enterobacteriaceae and appears not to be established locally.]]> Wed 04 Sep 2019 09:49:49 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49044 37 weeks) admitted to and treated in the neonatal unit at the Townsville University Hospital between March 2015 and March 2020. Neonatal sepsis was confirmed with positive neonatal blood culture. Data on neonatal birth/stay and maternal pregnancy were collected from the electronic medical records and neonatal database. Results: Data from 737 neonates who were admitted for treatment of EONS were analysed. Sixty % (426) reported maternal intrapartum fever, with 1.1% (5) of neonates developing blood culture-proven sepsis. Forty % did not report intrapartum fever (311), with 3% (9) of neonates developing sepsis. As such, the sensitivity and specificity of maternal fever are 1.14% and 97%, respectively. The positive predictive value was 35.7%, and the negative predictive value was 40.1%. Fourteen neonates developed EONS, and all of them were symptomatic. Seventy-eight % (334/426) of the women in the febrile group received epidural analgesia compared to 5% (16/311) in the afebrile group. Of the 95 neonates born to women with chorioamnionitis, one (1.0%) of the neonates born to women with chorioamnionitis developed sepsis. Conclusions: Intrapartum maternal fever is an unreliable predictor for EONS and leads to unnecessary antibiotic treatment. Symptoms in the neonate are a more reliable indicator of an ill neonate with blood culture-proven sepsis.]]> Wed 03 May 2023 13:55:00 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54526 Tue 27 Feb 2024 19:43:50 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:11516 Sat 24 Mar 2018 08:11:06 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10747 Sat 24 Mar 2018 08:08:20 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20873 Sat 24 Mar 2018 07:57:58 AEDT ]]>